The boundaries of the seven Missouri groundwater provinces depicted on a map of Missouri
Groundwater Provinces of Missouri include: Mississippi and Missouri River, Northeast, Northwest, St. Francois Mountains, Salem Plateau, Southeastern Lowlands, Springfield Plateau, and West-Central.

Missouri occupies an area of about 69,709 square miles, and the geologic conditions across the state vary greatly. There are more than a dozen major aquifers underlying various parts of the state. In some areas, three or more aquifers are present. In other areas where groundwater resources are poor, there may only be one aquifer or none at all. Because of these differences in geologic conditions, Missouri has been divided into eight major groundwater provinces: St. Francois Mountains, Salem Plateau, Springfield Plateau, Southeastern Lowlands, Northwestern Missouri, Northeastern Missouri, West-Central Missouri, and Missouri-Mississippi Alluvial Aquifers.

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Groundwater is contained in and moves through aquifers. Various definitions exist for the term aquifer, but generally an aquifer is defined as a saturated, permeable geologic unit that can transmit significant quantities of water under ordinary hydraulic gradients. Nearly all of the geologic formations in Missouri are capable of storing and discharging varying amounts of groundwater. However, not all of the units yield water in sufficient quantities to be considered important aquifers. Missouri's groundwater resources vary greatly across the state. They are closely tied to the geology of the various regions. The boundaries of the groundwater provinces are drawn based on several factors including aquifer characteristics, groundwater quality changes, and aquifer boundaries. 

Groundwater resources of Missouri are not evenly distributed. The amount of usable groundwater is far less in northern Missouri where only about 12 percent of Missouri's potable groundwater is found. The largest, most extensive aquifer is the Ozark Aquifer in the Salem and Springfield Plateau groundwater provinces. 

The distinction between an important aquifer and one which is not is often subjective, and to a great extent depends on location and perspective. A rural resident living in northwestern Missouri might be pleased to have a well that yields one to three gallons per minute (gpm). Since groundwater resources are generally poor in this area, and yields greater than this are uncommon, such a yield would be considered good by local standards. Another person living in central Missouri, where well yields are generally better, would likely be disappointed if his well yielded less than 15 gpm. For an irrigation system, public water supply, or an industry requiring substantial water, any quantity less than several hundred gallons per minute may not be considered significant.

Aquifers can be classified into two general types, unconfined aquifers and confined aquifers. In an unconfined aquifer, the water table forms the upper boundary of the aquifer. The water table is the surface between the unsaturated materials above the water table and the saturated materials below. The water level in a well drilled into an unconfined aquifer is the water table. A confined aquifer is bounded above and below by confining beds that have much lower permeabilities than the aquifer.

The water level in a tightly cased well drilled into a confined aquifer will usually be some distance above the top of the aquifer due to the head pressure on the water in the aquifer. If the water level in the well rises above land surface, it is termed a flowing artesian well.  

Aquifers are often named after the geologic formation that comprises them. Examples of this include the St. Peter Sandstone, the Missouri River alluvium, and the McNairy Formation. However, unless the specific geologic formation is hydrologically isolated from the units above and below it, it is generally best not to name the aquifer after the geologic unit. In Missouri, several geologic formations commonly comprise a single aquifer.  

Water termed “fresh” or “potable” refers to water containing less than 1,000 milligrams per liter (mg/L) of total dissolved solids, and less than 250 mg/L each of sulfate and chloride. These criteria are based, in part, on secondary water-quality standards used by the department for public water supplies. Aquifers that contain potable water in the Ozarks generally contain highly mineralized water in other places. Thus, the total volume of groundwater in storage statewide is somewhat greater than the volume of freshwater available to Missourians. 

In many areas of Missouri, the deep aquifer zones contain water that is of poor quality. In Missouri a natural feature known as the freshwater-saline water transition zone runs from approximately Joplin to Warrensburg, to Mexico to Troy to Festus. Groundwater contained in deeper aquifer zones south of the transition zone generally contains less than 1,000 mg/L total dissolved solids, and less than 250 mg/L of chloride and sulfate, and is generally potable without treatment. North of the transition zone, groundwater in the same aquifer zones becomes increasingly mineralized and contains excessive total dissolved solids and chloride, and may contain excessive sulfate which makes the water unpotable.